Rockland offers control cell lysates and nuclear extracts for use on SDS PAGE as standalone samples or in combination with antibodies on Western Blotting experiments.
Our ready-to-use whole cell lysates and nuclear extracts are derived from cell lines or tissues using highly advanced extraction protocols to ensure remarkably high quality, protein integrity and lot-to-lot reproducibility.
Lysates are generated either from whole cells which contain cell membrane, cytoplasmic and nuclear proteins, or nuclear extracts, which are predominantly proteins that originate in the nucleus. Control lysates may be from cells that are stimulated with insulin, doxorubicin, etoposide, nocodozole, TNFa or EGF. Lysates are also available from normal animal tissue derived from primary organs such as liver, heart and brain. Further, Rockland also offers a variety of lysates that contain over expressed proteins (tagged and untagged) that can serve as positive controls for antibody reactivity. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.