Oxford Gene Technology (OGT) has introduced CytoSure ISCA UPD 4 x 180k array which simultaneously detects DNA copy number variation (CNV) using ISCA 4 x 180k aCGH array, along with whole chromosome uniparental disomy (UPD) using SNP probes, on a single array.

OGT’s UPD detection capability (patent pending) has been enabled by multiple rounds of SNP probe selection and validation, targeting over 6000 SNPs with evenly distributed probes, resulting in informative content that allows confident detection of whole chromosome UPD.

OGT said that the combined ISCA-UPD array has been developed to ensure near identical labelling and hybridisation conditions to standard aCGH, which is unaltered by the use of any reference DNA.

The latest version of OGT’s CytoSure Interpret Software provides data processing tools and clear identification of regions with a loss of heterozygosity (LOH).

OGT Clinical and Genomic Solutions vice president James Clough said that they have carefully implemented UPD detection capabilities onto CytoSure ISCA 4 x 180k arrays, whilst avoiding wholesale changes to the protocol.

“Importantly, we have also made the interpretation of this additional data extremely intuitive via our CytoSure Interpret Software. As a result, our customers can now confidently detect both DNA copy number variation and whole chromosomal uniparental disomy using a single array,” Clough said.