Austria-based biotechnology company Lexogen has released a new whole transcriptome RNA-Seq library prep kit, CORALL RNA-Seq V2.

CORALL RNA-Seq V2 is based on the previously announced proprietary Displacement/Stop and Ligation technology.

It is a quick and flexible whole transcriptome sequencing kit with a wide input range and better performance, even for low input (down to 1 ng total RNA) and compromised samples, said Lexogen.

Unlike traditional WTS RNA-Seq, CORALL library preparation takes only six steps and 4.5 hours to complete.

CORALL does not need RNA fragmentation, making it ideal for processing highly degraded RNA or FFPE samples, the firm said.

CORALL RNA-Seq V2 improves the well-established technique by increasing the kit’s performance and resilience, as well as adding the ability to change the library size without utilising RNA fragmentation.

It has a new feature for difficult RNA-Seq applications like isoform discovery, transcript (re)-annotation, and the analysis of fusion transcripts.

The kit is provided as a standalone, as an mRNA-Seq bundle with poly (A) selection, and as a Total RNA-Seq bundle with RiboCop rRNA depletion for superior performance.

University of Bern next generation sequencing platform manager Dr Pamela Nicholson said: “We can support our researchers very well using Lexogen’s CORALL RNA-Seq Library preparation.

“We find the protocol to be straightforward and can generate very consistent libraries perfect for standard RNA-Seq projects—With CORALL RNA-Seq V2 we now have the choice to generate libraries with longer insert sizes […]. This is very useful, for example, for RNA-Seq for non-model organisms, for improved mapping for demanding projects, and for projects characterising isoforms.”

The whole-transcriptome analysis using RNA sequencing (RNA-Seq) allows researchers to identify the number of RNA molecules in a sample at the time of sampling.

The transcriptome is a highly dynamic biological characteristic, and RNA Sequencing unlocks a world of potential discovery and uses in sectors like gene expression studies.