Researcheres at the University of Pittsburgh Swanson School of Engineering needed needed two different microwell arrays that would support the injection molding of hydrogels and serve as “master molds” – original molds that he could replicate. In turn, these replicate molds would be used for microwell fabrication. To produce master molds like this, photolithography is often used. Typically, the production of master molds and masks requires specialized facilities and equipment. In this case study we learn how the University of Pittsburg researched used micro 3D printing to speed microwell array fabrication.