Product

Antibody Fragmentation Services

August 24, 2018

Rockland’s experience in working with antibody enzymatic cleavage allows us to process immunoglobulins into their constituent components.

In some assays, it is preferable to use only the antigen-binding (Fab) portion of the antibody to prevent the constant (Fc) portion from binding to cell surface receptors. For these applications, antibodies can be enzymatically digested using the enzyme papain to produce a Fab fragment and/or Fc fragment of the antibody or the enzyme pepsin to produce a F(ab’)2 fragment of the antibody. While Rockland offers secondary antibodies as Fab fragmentsand F(ab’)2 fragments, primary antibodies are digested into fragments as a custom service.