In-Fusion Cloning, developed by TBUSA, is a unique method that seamlessly joins together linear fragments of DNA in a single, 15-minute reaction.

The free, online primer design tool facilitates easy design and a smooth transition from planning to experimental work.

It was developed with TeselaGen specifically to cater to the requirements of In-Fusion Cloning and to support the wide range of cloning applications available when using In-Fusion technology, including multi-fragment cloning, mutagenesis, and general cloning.

As an expert in in silico DNA editing and design, TeselaGen was uniquely positioned to anticipate the complexities and nuances inherent in building this custom tool.

"In-Fusion technology is unique in its ability to provide scientists with a streamlined and efficient method to build any clone. Primer design is critical to PCR cloning success, and we developed this new tool to guide researchers through the process, step-by-step.

“With less time spent on experimental design and cloning, our customers can spend more time advancing their research," said Carol Lou, President of Takara Bio USA, Inc.

The In-Fusion Cloning Primer Design Tool responds in real time to user input and allows for easy visualization and planning of any In-Fusion Cloning project.

 It provides a final vector map and custom primer sequences, enabling seamless transition from design to bench work. No signup or software download is required, and all submitted sequences are protected with end-to-end encryption. No user data is stored.

In-Fusion cloning technology is flexible in kit format and cloning setup, and the primer design tool accommodates both of these aspects: The primer design function is compatible with vector linearization by inverse PCR or restriction digest, and with single- or multiple-insert cloning.

Additionally, In-Fusion Cloning contains protocols that accommodate lyophilized or liquid reagents and a variety of methods for purification of the PCR-generated inserts.

Dr. Andrew Farmer, Vice President of Research and Development at TBUSA, commented that "Concurrent with the release of this new tool, we have taken the opportunity to optimize primer specifications to improve the success of multiple-insert cloning approximately five-fold. This means that researchers can screen fewer colonies to obtain the right clone."

Dr. Michael Fero, Chief Executive Officer at TeselaGen, said, "TeselaGen is a platform that automates and optimizes protocol generation for DNA building and editing. We are very excited to open our platform to TBUSA users via this primer design tool. Takara Bio kits and protocols, including Clontech brand In-Fusion Cloning kits, are known for very high quality and close attention to detail.

“This collaboration is a natural one for us since making cloning easy and repeatable is a primary focus for us at TeselaGen."