Lucigen’s new Taq98 Hot Start 2X Master Mix amplifies difficult DNA templates, including targets with >70% GC content.

By replacing the Lucigen’s dNTPs in a PCR assay with CleanAmp dNTPs, a Hot Start reaction will be achieved.

The approach to new PCR provides a method to reduce mis-priming, primer dimer formation and other deleterious off-target effects in PCR.

Lucigen president Jeff Williams said the company has found that CleanAmp dNTPs improve the performance of a number of thermostable DNA polymerases, allowing for simple conversion of any assay to a Hot Start version.